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Brown TE (1998) Genetics: A Molecular Approach, 3rd edn. Chapman & Hall, London. Calladine, CR and Drew HR (2004) Understanding DNA: The Molecule and How it Works, 3rd edn. Academic Press, London Dale JW and Park SF (2004) Molecular Genetics of Bacteria, 4th edn. John Wiley & Sons, Chichester. Klug WS and Cummings MR (2005) Essentials of Genetics, 5th edn. Prentice Hall, Englewood Cliffs, NJ. Miller RV (1998) Bacterial gene swapping in nature. Scienti c American 278, 67 71. Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2004) Molecular Biology of the Gene, 5th edn. Benjamin Cummings, San Francisco, CA.

Adams WS, Rider L. Circular Polar Constellation providing continuous single or multiple coverage above a speci ed latitude. The Journal of Astronautical Sciences 1987;35(2): 155 192. Boorstin DJ. The discoverers. New York: Random House; 1983. Bostrom CO, Williams DJ. The space environment. Johns Hopkins APL Technical Digest, January March 1998;19(1). Danchik RJ. An overview of transit development. Johns Hopkins APL Technical Digest, January March 1998;19(1). Gardner AC. Navigation. UK: Hodder and Stoughton Ltd.; 1958. I and P. Taking the stars, celestial navigation from argonauts to astronauts. Virginia: The Mariners Museum Newport News; Florida: The Krieger Publishing Company; 1998. Kaplan ED, Hegarty C. Understanding GPS: principles and applications. 2nd ed. Artech House; 2006. Norwood, MA, USA. Kennedy GC, Crawford MJ. Innovations derived from the transit program. Johns Hopkins APL Technical Digest, January March 1998;19(1). Merrignan MJ et al. A re nement of the World Geodetic System 1984 reference frame. In: ION GPS 2002: Proceedings; Portland (OR); 2002. p 1519 1529. Parkinson BW, Spilker Jr. JJ. Global positioning system: theory and applications. American Institute of Aeronautics and Astronautics; 1996. Sobel D. Longitude. London: Fourth Estate Limited; 1996. Yionoulis SM. The transit satellite geodesy program. Johns Hopkins APL Technical Digest, January March 1998;19(1).

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Material Alginate (Keltone LV) L-Ascorbic acid-2-phosphate Bovine serum albumin CaCl2 Cell culture asks, 75 cm2 Cell culture asks, 25 cm2 Petri dishes, 10 cm Centrifuge tubes, 15 ml conical polypropylene Centrifuge tubes, 50 ml conical polypropylene Collagenase A Collagenase type II Cryotubes DMEM, 4.5 g/l glucose, containing pyruvate and glutamate DMEM, 1.0 g/l glucose, containing pyruvate and glutamate Dexamethasone Fetal bovine serum Filter, 500 ml, 0.22 m Filter, 250 ml, 0.22 m Filter (Steri ip) 50 ml, 0.22 m Filters, 40- m tube Fungizone antimycotic liquid Glutamine HCl Ham s F-12 nutrient medium Hanks balanced salt solution ITS-G Supplement (100 ) ITS + Premix Insulin-transferrin-sodium selenite supplement Opti-MEM 1 reduced-serum medium Penicillin-streptomycin liquid PBS, Mg2+ , Ca2+ -free Pronase NaCl Sodium pyruvate C6 H5 O7 Na3 2H2 O TGF- 1 Trypan Blue Trypsin-EDTA: 0.25% trypsin, 1 mM EDTA Ultra-low-attachment plates, 35 mm Catalog # N/A AC358610250 A 9647 C69-500 430641 430639 3160-100 352095 352079 1088785 LS004176 3471 10569-010 11885-084 D-4902 16000-044 SCGP UO5 RE SCGP UO2 RE SCGP 005 25 352340 15290-018 25-05-C1 A1445 31765-035 24020-117 41400-045 40352 1074547 31985-070 15140-122 14190-144 0165921 S671-500 11360-070 S279-500 240-B 15250-061 25200-056 3471 Supplier ISP Fisher Scienti c Sigma Fisher Scienti c Corning Corning Corning Becton Dickinson Becton Dickinson Roche Diagnostics Worthington Biochemical Corp CLP GIBCO Invitrogen GIBCO Invitrogen Sigma GIBCO Invitrogen Millipore Millipore Millipore Becton Dickinson GIBCO Invitrogen Cellgro Fisher GIBCO Invitrogen GIBCO Invitrogen GIBCO Invitrogen B-D Biosciences Roche Diagnostics GIBCO Invitrogen GIBCO Invitrogen GIBCO Invitrogen Roche Diagnostics Fisher Scienti c GIBCO Invitrogen Fisher Scienti c R&D Systems GIBCO Invitrogen GIBCO Invitrogen Costar

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Different sources of location information have their peculiarities GSM cell tower identi ers give coarse estimates of the location; the numbering of the identi ers is seemingly random and, even though operators allow the obtaining of the current cell identi er, they normally do not offer services to convert cell identi ers into geographic locations On the other hand, GSM cell tower identi er information is available also indoors, whereas GPS measurements are not GPS signals may also be blocked in an outdoor environment by buildings and trees Finally, WiFi access point information can be used for positioning when the locations of access points are known [63] Regardless of the source of location information, the raw measurements are usually meaningless to the user As a consequence, much work has been conducted on identifying signi cant locations from the raw data.

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